It seems that only the normals of the hub and air cap are correct. Is there something wrong with the tires?
The picture below is the status in blender.
Check your normals on the high and low tyre in Blender, one or the other could be the problem.
All exterior faces should be blue, if you see any red, in edit mode press A to select everything then SHIFT+N to recalculate the normals.
You will then need to save and re-export the model.
To add on to Adrian, your last screenshot makes it seem like your Highpoly Tread object has flipped-normals which could be causing issues. Following his advice, I think we may be able to fix this easily!
If you tackle that and it still is presenting issues, please send a screenshot of the Bake settings you used in Substance Painter, chances are that's another area that could be causing issue. :)